Life & Medical Sciences: Genetics: Books and Magazines

Molecular Cloning: A Laboratory Manual (3-Volume Set)
by Joseph Sambrook, David W. Russell, Joe Sambrook
Peter MacCallum Cancer Institute, Melbourne, Australia. Updated text includes revised protocols and new material for students in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Describes techniques, cDNA cloning and more. Softcover, hardcover also available. DNLM: Cloning, Molecular--Laboratory Manuals

The Genetics and Biology of Sex Determination - No. 244
Although nature employs a wide variety of sex-determining mechanisms, it is only comparatively recently that the tools have become available for these to be explored at the molecular and cellular levels. A major landmark was the discovery in 1990 of the Sry gene and the subsequent demonstration of its key role in triggering male sex determination in transgenic mice. Since that time, while other key genes in the sex-determining pathway have been uncovered, the story remains far from complete. This ground-breaking book draws together leading researchers to review and discuss the most recent advances in the understanding of the molecular genetic pathways of sex determination. Coverage includes the genetics and biochemistry of mammalian sex determination, comparisons with other modes of sex determination, and consideration of the biology of sexual development and of the evolution of sex-determining mechanisms. A major theme running through several chapters is the value of comparative studies of sex-determining mechanisms in different species. In addition, clinical aspects and the genetic analysis of anomalies of human sexual development are also addressed. This exciting book reviews much new and exciting work in this area and identifies promising new research directions.


Analysis of Genes and Genomes
Introduction to the theoretical and practical basis of genetic engineering, gene cloning and molecular biology. All aspects of genetic engineering in the post-genomic era are covered, beginning with the basics of DNA structure and DNA metabolism. Using an example-driven approach, the fundamentals of creating mutations in DNA, cloning in bacteria, yeast, plants and animals are all clearly presented. Newer technologies such as DNA macro and macroarrays, proteomics and bioinformatics are introduced in later chapters helping students to analyse and understand the vast amounts of data that are now available through genome sequence and function projects. Aimed at students with a basic knowledge of the molecular side of biology, this will be invaluable to those looking to better understand the complexities and capabilities of these important new technologies. A modern post-genome era introduction to key techniques used in genetic engineering. An example driven past-to-present approach to allow the experiments of today to be placed in an historical context Beautifully illustrated in full colour throughout. Associated website including updates, additional content and illustrations

Table of Contents: 1 – DNA: structure and function. 1.1 Nucleic acid is the material of heredity. 1.2 Structure of nucleic acids. 1.3 The double helix. 1.4 Reversible denaturing of DNA. 1.5 Structure of DNA in the cell. 1.6 The eukaryotic nucleosome. 1.7 The replication of DNA. 1.8 DNA polymerases. 1.9 The replication process. 1.10 Recombination. 1.11 Genes and genomes. 1.12 Genes within a genome. 1.13 Transcription. 1.14 RNA processing. 1.15 Translation. 2 – Basic techniques in gene analysis. 2.1 Restriction enzymes. 2.2 Joining DNA molecules. 2.3 The basics of cloning. 2.4 Bacterial transformation. 2.5 Gel electrophoresis. 2.6 Nucleic acid blotting. 2.7 DNA purification. 3 – Vectors. 3.1 Plasmids. 3.2 Selectable markers. 3.3 l Vectors. 3.4 Cosmid vectors. 3.5 M13 vectors. 3.6 Phagemids. 3.7 Artificial chromosomes. 4 – Polymerase chain reaction. 4.1 PCR reaction conditions. 4.2 Thermostable DNA polymerases. 4.3 Template DNA. 4.4 Oligonucleotide primers. 4.5 Primer mismatches. 4.6 PCR in the diagnosis of genetic disease. 4.7 Cloning PCR products. 4.8 RT-PCR. 4.9 Real-time PCR. 4.10 Applications of PCR. 5 – Cloning a gene. 5.1 Genomic libraries. 5.2 cDNA libraries. 5.3 Directional cDNA cloning. 5.4 PCR-based libraries. 5.5 Subtraction libraries. 5.6 Library construction in the post-genome era. 6 – Gene identification. 6.1 Screening by nucleic acid hybridization. 6.2 Immunoscreening. 6.3 Screening by function. 6.4 Screening by interaction. 6.5 Phage display. 6.6 Two-hybrid screening. 6.7 Other interaction screens – variations on a theme. 7 – Creating mutations. 7.1 Creating specific mutations. 7.2 Primer extension mutagenesis. 7.3 Strand selection methods. 7.4 Cassette mutagenesis. 7.5 PCR-based mutagenesis. 7.6 QuikChange mutagenesis. 7.7 Creating random mutations in specific genes. 7.8 Protein engineering. 8 – Protein production and purification. 8.1 Expression in E. coli. 8.2 Expression in yeast. 8.3 Expression in insect cells. 8.4 Expression in higher eukaryotic cells. 8.5 Protein purification. 9 – Genome sequencing projects. 9.1 Genomic mapping. 9.2 Genetic mapping. 9.3 Physical mapping. 9.4 Nucleotide sequencing. 9.5 Genome sequencing. 9.6 The Human Genome Project. 9.7 Finding genes. 9.8 Gene assignment. 9.9 Bioinformatics. 10 – Post-genome analysis. 10.1 Global changes in gene expression. 10.2 Protein function on a genome-wide scale. 10.3 Knock-out analysis. 10.4 Antisense and RNA interference (RNAi). 10.5 Genome-wide two-hybrid screens. 10.6 Protein-detection arrays. 10.7 Structural genomics. 11 – Engineering plants. 11.1 Cloning in plants. 11.2 Commercial exploitation of plant transgenics. 11.3 Ethics of genetically engineered crops. 12 – Engineering animal cells. 12.1 Cell culture. 12.2 Transfection of animal cells. 12.3 Viruses as vectors. 12.4 Selectable markers and gene amplification in animal cells. 12.5 Expressing genes in animal cells. 13 – Engineering animals. 13.1 Pronuclear injection. 13.2 Embryonic stem cells. 13.3 Nuclear transfer. 13.4 Gene therapy. 13.5 Examples and potential of gene therapy.


Animal Transgenesis and Cloning


Handbook of Statistical Genetics, Second Edition

Genome Transcriptome and Proteome Analysis


Molecular Genetics of Bacteria, Fourth Edition


The Geometry of Genetics


Phylogenetics: The Theory and Practice of Phylogenetic Systematics


Microbial Functional Genomics


Breakthrough: The Race to Find the Breast Cancer Gene


Clinical Cancer Genetics: Risk Counseling and Management


Applied Molecular Genetics


Approaches to Gene Mapping in Complex Human Diseases


Unraveling DNA


A Guide to Genetic Counseling

Architecture of Eukaryotic Genes


The Science and Ethics of Engineering the Human Germ Line: Mendel's Maze


Essentials of Medical Genomics


Pharmacogenomics: Social, Ethical, and Clinical Dimensions


Genetics and the Logic of Evolution


Genomes , 2nd Edition

Principles of Genetics, Study Guide, 3rd Edition

Principles of Genetics, Take Note!, 3rd Edition


Encyclopedic Dictionary of Genetics, Genomics, and Proteomics, 2nd Edition


Essentials of Medical Genomics


Exploration and Analysis of DNA Microarray and Protein Array Data


Positional Cloning by Exon Trapping and cDNA Selection


Clinical Genetics: A Short Course


Management of Genetic Syndromes


What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice


Protein Sequencing and Identification Using Tandem Mass Spectrometry


Human Molecular Genetics, 2nd Edition


Psyche and Helix: Psychological Aspects of Genetic Counseling


Genomic Imprinting and Uniparental Disomy in Medicine: Clinical and Molecular Aspects


Genetics and the Search for Modern Human Origins

Problems and Solutions for Strachan and Read's Human Molecular Genetics, 2nd Edition

Human Molecular Genetics, Textbook and Problems Set, 2nd Edition


Modern Microbial Genetics, 2nd Edition


Handbook of Comparative Genomics: Principles and Methodology


An Introduction to Molecular Medicine and Gene Therapy


Principles of Genetics, 3rd Edition

Mouse Models of Cancer

Principles of Genetics, Third Edition, packaged with the Gene View CD-ROM 1.0


The Science and Ethics of Engineering the Human Germ Line: Mendel's Maze


Biostatistical Genetics and Genetic Epidemiology


From Genes to Genomes: Concepts and Applications of DNA Technology

Molecular Analysis and Genome Discovery


Mapping the Code: The Human Genome Project and the Choices of Modern Science


Understanding Genetics: A Molecular Approach


From Genome to Therapy: Integrating New Technologies with Drug Development - No. 229


Genetic Techniques for Biological Research: A Case Study Approach


Working with Animal Chromosomes, 2nd Edition


Genetic Analysis of Tumour Suppression - No. 142


Genetics of Criminal and Antisocial Behaviour - No. 194


Variation in the Human Genome - Symposium No. 197


Transgenic Plants: A Production System for Industrial and Pharmaceutical Proteins


Epigenetics - No. 214


Genetics and Tuberculosis - No. 217


An Introduction to Human Molecular Genetics: Mechanisms of Inherited Diseases

Gene Function & Disease


The Dictionary of Gene Technology: Genomics, Transcriptomics, Proteomics, 2nd Edition


Pharmacogenomics: The Search for Individualized Therapies

American Journal of Medical Genetics

Comparative and Functional Genomics

Genetic Epidemiology

Human Mutation

The Journal of Gene Medicine

Yeast
Yeast is a journal published for the international community of yeast researchers. It contains original research articles, major and minor reviews and short communications on all aspects of Saccharomyces and other yeast genera. The topics covered include: genetics; genomics; molecular biology; cell and developmental biology; biochemistry; physiology; pathobiology; biotechnology; ecology; taxonomy; and evolution. The journal contains three special sections: Yeast Sequencing Reports, Yeast Mapping Reports, and Yeast Functional Analysis Reports. These deal, respectively, with the systematic analysis of the sequences, placement, and function of genes. In addition, the journal promotes the collegiate activities of the yeast research community by publishing announcements of Meetings and Courses, as well as the Abstracts of major conferences. Yeast also has a Current Awareness on Yeast section that provides a comprehensive survey of recent publications on yeasts. Thus, Yeast focusses on the most significant developments of research with unicellular fungi and is essential reading for those wishing to keep up to date with this rapidly moving field.

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